285 research outputs found
Probing subtle fluorescence dynamics in cellular proteins by streak camera based Fluorescence Lifetime Imaging Microscopy
We report the cell biological applications of a recently developed
multiphoton fluorescence lifetime imaging microscopy system using a streak
camera (StreakFLIM). The system was calibrated with standard fluorophore
specimens and was shown to have high accuracy and reproducibility. We
demonstrate the applicability of this instrument in living cells for measuring
the effects of protein targeting and point mutations in the protein sequence
which are not obtainable in conventional intensity based fluorescence
microscopy methods. We discuss the relevance of such time resolved information
in quantitative energy transfer microscopy and in measurement of the parameters
characterizing intracellular physiology
Structure of incommensurate gold sulfide monolayer on Au(111)
We develop an atomic-scale model for an ordered incommensurate gold sulfide (AuS) adlayer which has previously been demonstrated to exist on the Au(111) surface, following sulfur deposition and annealing to 450 K. Our model reproduces experimental scanning tunneling microscopy images. Using state-of-the-art Wannier-function-based techniques, we analyze the nature of bonding in this structure and provide an interpretation of the unusual stoichiometry of the gold sulfide layer. The proposed structure and its chemistry have implications for related S-Au interfaces, as in those involved in self-assembled monolayers of thiols on Au substrates
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Dislocation nucleation in bcc Ta single crystals studied by nanoindentation
The study of dislocation nucleation in closed-packed metals by nanoindentation has recently attracted much interest. Here, we address the peculiarities of the incipient plasticity in body centered cubic (bcc) metals using low index Ta single-crystals as a model system. The combination of nanoindentation with high-resolution atomic force microscopy provides us with experimental atomic-scale information on the process of dislocation nucleation and multiplication. Our results reveal a unique deformation behavior of bcc Ta at the onset of plasticity which is distinctly different from that of closed-packed metals. Most noticeable, we observe only one rather than a sequence of discontinuities in the load-displacement curves. This and other differences are discussed in context of the characteristic plastic deformation behavior of bcc metals
Breaking the Screen: Interaction Across Touchscreen Boundaries in Virtual Reality for Mobile Knowledge Workers.
Virtual Reality (VR) has the potential to transform knowledge work. One
advantage of VR knowledge work is that it allows extending 2D displays into the
third dimension, enabling new operations, such as selecting overlapping objects
or displaying additional layers of information. On the other hand, mobile
knowledge workers often work on established mobile devices, such as tablets,
limiting interaction with those devices to a small input space. This challenge
of a constrained input space is intensified in situations when VR knowledge
work is situated in cramped environments, such as airplanes and touchdown
spaces.
In this paper, we investigate the feasibility of interacting jointly between
an immersive VR head-mounted display and a tablet within the context of
knowledge work. Specifically, we 1) design, implement and study how to interact
with information that reaches beyond a single physical touchscreen in VR; 2)
design and evaluate a set of interaction concepts; and 3) build example
applications and gather user feedback on those applications.Comment: 10 pages, 8 figures, ISMAR 202
Optical spin-to-orbital angular momentum conversion in inhomogeneous anisotropic media
We demonstrate experimentally an optical process in which the spin angular
momentum carried by a circularly polarized light beam is converted into orbital
angular momentum, leading to the generation of helical modes with a wavefront
helicity controlled by the input polarization. This phenomenon requires the
interaction of light with matter that is both optically inhomogeneous and
anisotropic. The underlying physics is also associated with the so-called
Pancharatnam-Berry geometrical phases involved in any inhomogeneous
transformation of the optical polarization
ALD Functionalized Nanoporous Gold: Thermal Stability, Mechanical Properties, and Catalytic Activity
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